SKU: 98634207433

Rat MT-1 (Metallothionein-1) ELISA Kit

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Description

Rat MT-1 (Metallothionein-1) ELISA KitThe Rat MT 1 (Metallothionein 1) ELISA Kit measures MT1 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti MT1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.

The Rat MT-1 (Metallothionein-1) ELISA Kit measures MT1 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti MT1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with MT1 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of MT1 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 98634207433

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Oj_0
Los Angeles, US
★★★★★ 5
Great for a Ralph Wiggum fan!
Format: Hardcover
Bought this for a friend for his bday and he loved it. Huge Simpson's fan,favorite character is Ralph, loved how the book was filled with random facts and weird thoughts.
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Reviewed in the United States on January 28, 2020
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Cory L. Dudak
Massapequa, US
★★★★★ 5
Funny and unexpected
Format: Hardcover
I bought this book for my girlfriend who is a big Ralph Wiggum fan. When it arrived, I was shocked to see what a thorough exploration of the character it was. I'm not saying it's all canon in terms of the show, as i saw some complaints that there was stuff in this book that wouldn't make sense to the Ralph character (such as his love for Lisa) due to them "breaking up" in a past episode, but come on, that's a hogwash complaint. The whole point of this book is to entertain, and it does so in spades, so it comes highly recommended. And this is coming from a huge 'The Simpsons' fan himself. Oh, and by the way, my girlfriend loved it, too.
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Reviewed in the United States on January 14, 2014
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Tee D
Grantham, US
★★★★★ 5
amazing
Format: Hardcover
i purchased this for my son when he turned 13. He loves the quotes and the book. We both have gotten countless laughs from it. Definitely an excellent purchase.
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Reviewed in the United States on April 30, 2021
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Amy McDonald
Houston, US
★★★★★ 5
my 12yo has not enjoyed a book so much in years
Format: Hardcover
this book is smaller than I thought it would be but it was so jam packed full of information ,my 12yo has not enjoyed a book so much in years , we had to tag team reading it to him as he did not want to stop , it was so funny as well
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Reviewed in the United States on June 19, 2016
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Kindle Customer
Fort Morgan, US
★★★★★ 5
Fun advise
Format: Hardcover
Great advise. My son has been a Simpsons fan since the Butterfinger commercials. A no brainer for a father's day gift.
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Reviewed in the United States on August 19, 2020

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