PGD2 (Prostaglandin D2) ELISA Kit
SKU: 74415672811

PGD2 (Prostaglandin D2) ELISA Kit

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Description

PGD2 (Prostaglandin D2) ELISA KitThe PGD2 (Prostaglandin D2) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with PGD2. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to PGD2. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme substrate

The PGD2 (Prostaglandin D2) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PGD2. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to PGD2. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PGD2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 74415672811

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