SKU: 66574060581

Rabbit HIF1A (Hypoxia Inducible Factor 1 Alpha) QuickTest ELISA Kit

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Description

Rabbit HIF1A (Hypoxia Inducible Factor 1 Alpha) QuickTest ELISA KitThe Rabbit HIF1A (Hypoxia Inducible Factor 1 Alpha) QuickTest ELISA Kit measures HIF1A in Rabbit Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and

The Rabbit HIF1A (Hypoxia Inducible Factor 1 Alpha) QuickTest ELISA Kit measures HIF1A in Rabbit Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into individual wells. If the sample contains HIF1a, a capture antibody-HIF1a-biotin-detection antibody complex was formed. After incubation, unbound conjugates were removed by wash buffer. HRP-Streptavidin was added. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of HIF1a in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 66574060581

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Blissfulsan
Port Orchard, US
★★★★★ 5
Timely delivery.
Format: Paperback
I got the ordered item within the time. The book was in good shape
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Reviewed in the United States on May 4, 2025
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Muni
Los Angeles, US
★★★★★ 5
Worth it
Format: Paperback
Excellent, needed for class
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Reviewed in the United States on June 11, 2021
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Amazon Customer
Omaha, US
★★★★★ 5
Another fine Piece
As with Bowlbys' other works, this classic furthers the hypothesis of negative emotional influence on the continued development of humans as we integrate with our social environments. I liked it...in fact, liked all of Bowlbys' writings.
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Reviewed in the United States on September 12, 2013
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fatimah
Fort Morgan, US
★★★★★ 5
Five Stars
A MUST HAVE BOOK FOR anyone interested in parenting! or have kids.
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Reviewed in the United States on March 20, 2015
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Ng Wai Yin
Birmingham, US
★★★★★ 4
A Groundbreaking Classic on Young Child Development
Format: Paperback
This first volume of John Bowlby's trilogy on Attachment and Loss expands and builds upon an article he published in 1958 in the International Journal of Psycho-Analysis titled "The Nature of the Child's Tie to His Mother", which is perhaps a more telling title than that of the book itself. Attachment, as a technical term in behavioural biology, is first used in describing instinctive mother-following behaviours of young mammals and birds (first observed and reported in delightful accounts by the Austrian ethologist Konrad Lorenz in the 1930's). By comparing data collected during and after the Second World War by childcare workers and researchers in U.K. and North America, Bowlby found a striking common pattern of distressed behaviours among young children between the ages of one and three when separated from mother for an extended period: first in Protest, then Despair and finally Detachment - a psychopathological state when a child becomes socially uninitiated and withdrawn, even to his returning mother. Bowlby then postulates that physical proximity to a mother-figure is essential to a child's development of cognitive capacities, especially during a sensitive period around six months to two years after birth. Attachment behaviours, like those of young mammals and birds, are present in the human baby too. This has since led to a blossoming of research activities in development psychology and psychoanalysis, as well as neurophysiology recently, which supplies much fresh evidence about the young brain and its phenomenal maturing in the first two years. Attachment theory has since contributed significantly to understanding of our own selves, informed the age-old philosophical debate on nature or nurture, and brought our attention to fundamental issues in child-rearing such as sensitive periods of development, the difference between attachment (conducive to security) and dependence (symptomatic of insecurity), the distinction between anxiety from separation and fear of the unfamiliar, etc. This new edition is a timely reprint of a classic account of attachment theory as formulated by the originator. While primarily an academic work, with a few chapters deemed more for an academic jury (about Freud and instinctive behaviours, etc.), it is mostly very readable, and certainly captivating to those with access to young babies, of whose behaviours are given an enlightening perspective. This volume focuses on attachment, with subsequent volumes on its loss in temporary and permanent terms respectively.
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Reviewed in the United States on September 4, 2003

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