Porcine PUMA (P53 Upregulated Modulator of Apoptosis) ELISA Kit
SKU: 17363769877

Porcine PUMA (P53 Upregulated Modulator of Apoptosis) ELISA Kit

Sale price$202.50 Regular price$225.00
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Description

Porcine PUMA (P53 Upregulated Modulator of Apoptosis) ELISA KitThe Porcine PUMA (P53 Upregulated Modulator of Apoptosis) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Porcine PUMA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine PUMA and Avidin Horseradish Peroxidase (HRP) conjugate are added

The Porcine PUMA (P53 Upregulated Modulator of Apoptosis) ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine PUMA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine PUMA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine PUMA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine PUMA. You can calculate the concentration of Porcine PUMA in the samples by comparing the OD of the samples to the standard curve.

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SKU: 17363769877

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